Against the backdrop of the vigorous development of modern animal husbandry, the extensive use of antibiotics has given rise to a severe problem—antibiotic residues. In particular, residues of fluoroquinolone antibiotics such as ciprofloxacin in milk have become a major concern in the field of food safety. Long-term intake of milk containing such antibiotic residues poses numerous potential risks to human health, including the induction of drug resistance and interference with the normal growth and development of children.

To safeguard the food safety line, the timely and accurate detection of antibiotic residues in milk is particularly crucial. Traditional detection methods, such as high-performance liquid chromatography, although featuring high sensitivity, involve cumbersome operating procedures and are time-consuming, making it difficult to meet the practical demand for rapid detection.

Against this background, Jingyi Optoelectronics, in collaboration with a professional research team, has made unremitting explorations and R&D efforts and successfully developed a rapid detection method for ciprofloxacin in milk based on a mini Raman spectrometer, injecting new vitality into food safety supervision.

The mini Raman spectrometer is a high-performance detection device with ultra-fluorescence suppression. Based on the ultra-high fluorescence suppression effect inherent in the 1064nm excitation light, it is especially suitable for the detection of high-fluorescence products. This characteristic endows it with unique advantages in the detection of complex matrices such as milk.

The technical principle of this detection method is as follows: first, the milk sample is pretreated by protein precipitation, then the obtained supernatant is fully mixed with gold nanoparticles, and subsequently detection is carried out using Surface-Enhanced Raman Scattering (SERS) technology. During the detection process, the high-efficiency Raman spectrum recognition algorithm built into the mini Raman spectrometer plays a key role. It can perform non-discriminatory detection of substances and easily identify the target substance—ciprofloxacin. Through a large number of experiments, researchers have found that the characteristic peak of ciprofloxacin appears at 1266 cm⁻¹, and its intensity exhibits a good linear relationship with the concentration of ciprofloxacin. Practical tests have shown that the limit of detection (LOD) of this method can reach 0.25 μg/mL, which is equivalent to the technical level of professional laboratories and fully meets the stringent requirements of actual detection.

Compared with traditional detection methods, the detection method based on the mini Raman spectrometer has distinct advantages.

First, it is fast and efficient—the entire detection process takes only 10 minutes, which greatly improves detection efficiency and can timely provide accurate data for food safety supervision.

Second, it is easy to operate—without cumbersome sample preprocessing steps, ordinary operators can easily master it after simple training.

Third, it features strong portability—the mini Raman spectrometer has an extremely small overall size and weighs less than 1.2kg, making it highly portable for on-site detection anytime and anywhere in various places such as customs, laboratories, workshops, warehouses and docks.

Fourth, it is cost-effective—compared with traditional detection methods, this method has obvious advantages in terms of instrument purchase, consumable use and labor costs, making it more suitable for large-scale promotion and application.

The mini Raman spectrometer not only performs excellently in the detection of ciprofloxacin in milk but also has broad expansion space in its application fields. This method is expected to be further extended to the detection of other antibiotics, such as enrofloxacin and norfloxacin, providing strong support for the comprehensive protection of food safety.

Jingyi Optoelectronics has always been committed to providing customers with comprehensive technical support and services, including the establishment of spectral libraries, method development and validation, and IQ/OQ/PQ certification support. Relying on its strong technical strength and professional service team, Jingyi Optoelectronics will continue to play an important role in the fields of public safety, food safety, pharmaceutical safety and so on, enabling rapid and non-destructive detection technology to truly achieve "Everything is under your control", and make greater contributions to safeguarding public health and social stability.

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